Practical Essentials of Solid-Phase Micro-Extraction

Module 4 of the Practical Essentials of Sample Preparation course.

This course is spent learning the theory behind Solid-Phase Micro-Extraction (SPME), how to modify samples to improve results, how to create SPME methods, analyse samples and the parameters which can be optimised. This course will cover Headspace-SPME and Immersion-SPME, SPME phases, SPME instrumentation & set up, maintenance and troubleshooting. It will introduce related techniques like SBSE, SPE-TD and SPDE.

Learning outcomes

• You will learn about how to use SPME as an automated sample preparation technique for GC & GC-MS / HPLC & LC-MS in great detail
• You will learn the fundamental theory of SPME
• You will learn about the types of samples that can be analysed using SPME
• You will learn about the various parameters that can be optimised for the technique to improve method robustness and sensitivity
• You will learn about the individual steps and processes of SPME analysis
• You will learn the frequent maintenance and common troubleshooting problems in SPME analysis
• You will learn SPME tips and tricks
• You will learn about the benefits of using SPME in the analysis of your samples

Prerequisites

This course requires good knowledge of GC or GC-MS, HPLC or LC-MS. The course is applicable to both techniques.