Understanding UHPLC

This course explores the key principles and practical considerations of Ultra High Performance Liquid Chromatography (UHPLC) to facilitate robust methods suitable for routine analysis. Covering essential topics such as system hardware requirements and differences, column design and chemistry, flow paths, and performance-critical parameters like resolution, efficiency, selectivity, and backpressure.

A strong emphasis is placed on practical considerations, unique to UHPLC, that cause issues with method robustness in routine applications. These include considerations during method development, such as mobile phase composition, column chemistry and dimensions, to day-to-day considerations such as differences in hardware and preparation of reagents and samples.

With a clear focus on practical applications, this training enhances understanding and confidence in UHPLC, helping participants troubleshoot effectively, optimize methods, and make better-informed decisions across the entire chromatographic workflow. By building both theoretical insight and practical skills, the course promotes greater independence, reduced errors, and improved return on analytical investment.

The cost of the course is available upon request from the training provider.

Prerequisites

There are no prerequisites for this course. For more information about the course, please click 'find out more' below.