Issue 6, 2006
From the journal:

Analyst

An impedance array biosensor for detection of multiple antibody–antigen interactions

Xiaobo Yu,a   Renji Lv,b   Zhenqiu Ma,b   Zhihong Liu,b   Yanhong Hao,a   Qingzhang Lia  and  Danke Xu*b  

* Corresponding authors

a College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China

b Department of Biochemistry, Beijing Institute of Radiation Medicine, Beijing Proteomics Research Center, Beijing, 100850, People's Republic of China
E-mail: xudk@nic.bmi.ac.cn
Fax: +86-10-66932225
Tel: +86-10-66932225

Abstract

Electrochemical impedance spectroscopy (EIS) combined with a gold electrode array was developed to detect multiple antibody–antigen interactions. Hepatitis B surface antigen (HBsAg), as a model sample, was employed to evaluate the characteristics of the biosensor. The array was fabricated by immobilizing antibodies on the self-assembled molecules surface of the electrodes. The surface characteristics of the array during the binding process including the antibody–antigen conjugation and the sandwich complex with HRP-labeled antibody, as well as the precipitation layer, were characterized by atomic force microscopy (AFM) and electrochemical impedance spectroscopy, respectively. A linear relationship between electron-transfer resistance and the concentrations of HBsAg ranged from 10 pg ml−1 to 1 ng ml−1 and the detection limit of 10 pg ml−1 was obtained. 100 pg ml−1 antigen samples, such as rat IgG, HBsAg and HBeAg, as well as the antigen mixture, were incubated with the relative antibody-modified electrodes on the array. No obvious cross-talk reaction was observed. All these results confirm the feasibility of applying electrochemical impedance spectroscopy to the electrode array.

Graphical abstract: An impedance array biosensor for detection of multiple antibody–antigen interactions

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Article information

DOI
https://doi.org/10.1039/B517148B
Article type
Paper
Submitted
05 Dec 2005
Accepted
30 Mar 2006
First published
13 Apr 2006

Analyst, 2006,131, 745-750

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An impedance array biosensor for detection of multiple antibody–antigen interactions

X. Yu, R. Lv, Z. Ma, Z. Liu, Y. Hao, Q. Li and D. Xu, Analyst, 2006, 131, 745 DOI: 10.1039/B517148B

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