Issue 9, 2007
From the journal:

Analyst

Multiplexed detection of ions and mRNA expression in single living cells

Colin D. Medley,a   Hui Lin,a   Hillary Mullins,b   Richard J. Rogersb  and  Weihong Tan*a  

* Corresponding authors

a Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, USA
E-mail: tan@chem.ufl.edu
Fax: 352-846-2410
Tel: 352-846-2410

b Department of Anesthesiology, University of Florida College of Medicine, Gainesville, FL, USA

Abstract

In order to push forward into new areas of medical and biological research, new techniques must be developed that will enable a complex investigation into cellular processes. This involves investigating not only the different expression levels inside of a cell but also the ability to analyze how those expression levels are connected to one another. In order to accomplish this level of exploration, different types of analytes must be investigated simultaneously inside of single cells, thereby allowing their expression levels to be directly compared. To accomplish this, we have developed a method of detecting and monitoring mRNA expression levels and ion concentrations simultaneously inside of the same single cell. We have utilized this technique in studying the effects of an anti-cancer agent on human breast carcinoma cells. Using this approach, we are able to shed light onto the complex connections between genes and ions inside the cell that is not possible with any other existing technique.

Graphical abstract: Multiplexed detection of ions and mRNA expression in single living cells

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Article information

DOI
https://doi.org/10.1039/B701485F
Article type
Paper
Submitted
31 Jan 2007
Accepted
06 Jul 2007
First published
30 Jul 2007

Analyst, 2007,132, 885-891

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Multiplexed detection of ions and mRNA expression in single living cells

C. D. Medley, H. Lin, H. Mullins, R. J. Rogers and W. Tan, Analyst, 2007, 132, 885 DOI: 10.1039/B701485F

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