Issue 7, 2009
From the journal:

Analyst

Probing inhibitory effects of destruxins from Metarhizium anisopliae using insect cell based impedance spectroscopy: inhibition vs chemical structure†

Keith B. Male,a   Yew-Min Tzeng,*b   Johnny Montes,a   Bing-Lan Liu,b   Wan-Chun Liao,b   Amine Kamena  and  John H. T. Luong*ac  

* Corresponding authors

a Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada

b Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, ROC

c Analytical and Biological Research Facility (ABCRF), Department of Chemistry, University College Cork, Cork, Ireland

Abstract

A noninvasive technique based on electric cell-substrate impedance sensing (ECIS) was demonstrated for on-line probing inhibitory effects of five destruxins on Spodoptera frugiperda Sf9 insect cells. Such chemically structurally similar cyclic hexadepsipeptides, were isolated and purified from the fungus Metarhizium anisopliae. Based on a response function, the inhibitory effect of the destruxins was established from determining the half-inhibition concentration (ECIS50), i.e., the level at which 50% inhibition of the cell response was obtained. Probing by cell based impedance spectroscopy indicated that only a slight change in their chemical structures provoked a significant effect on inhibition. Destruxin B was most inhibitory but replacement of a single methyl group with hydrogen (destruxin B2) or addition of a hydroxyl group (destruxin C) significantly reduced the inhibition. The removal of one methyl group and one hydrogen (destruxin A) lowered the inhibitory effect even more whereas the formation of an epoxy ring (destruxin E) in the structure nullified the inhibitory effect.

Graphical abstract: Probing inhibitory effects of destruxins from Metarhizium anisopliae using insect cell based impedance spectroscopy: inhibition vs chemical structure

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B822133B
Article type
Paper
Submitted
09 Dec 2008
Accepted
31 Mar 2009
First published
15 Apr 2009

Analyst, 2009,134, 1447-1452

Permissions

Request permissions

Probing inhibitory effects of destruxins from Metarhizium anisopliae using insect cell based impedance spectroscopy: inhibition vs chemical structure

K. B. Male, Y. Tzeng, J. Montes, B. Liu, W. Liao, A. Kamen and J. H. T. Luong, Analyst, 2009, 134, 1447 DOI: 10.1039/B822133B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements