Issue 7, 2004
From the journal:

Physical Chemistry Chemical Physics

Interactions between bovine serum albumin layers adsorbed on different substrates measured with an atomic force microscope†

Juan José Valle-Delgado,*a   José Antonio Molina-Bolívar,b   Francisco Galisteo-González,a   María José Gálvez-Ruiz,a   Adam Feilerc  and  Mark Rutlandc  

* Corresponding authors

a Grupo de Física de Fluidos y Biocoloides, Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain
E-mail: jjvalle@ugr.es
Fax: +34 958 243214
Tel: +34 958 246175

b Departamento de Física Aplicada II, Escuela Universitaria Politécnica, Universidad de Málaga, 29013 Málaga, Spain

c Department of Chemistry, Surface Chemistry, Royal Institute of Technology and Institute for Surface Chemistry, 100 44 Stockholm, Sweden

Abstract

By the aid of an atomic force microscope (AFM) and the colloid probe technique, the interaction forces between bovine serum albumin (BSA) layers adsorbed on different substrates (silica and polystyrene) have been measured directly as a function of pH and salt concentration. Electrostatic and steric forces dominate the interactions at low salt concentrations. At high salt concentrations, when electrostatic interactions are screened, a very strange behaviour is found as a function of pH. The behaviour around the i.e.p. of the protein is also very striking: the interaction is attractive at low salt concentration, but it is repulsive at high salt concentration. These results could be explained if the presence of hydration forces is assumed. Theoretical predictions including a hydration term in the DLVO theory fit the experimental results satisfactorily.

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Article information

DOI
https://doi.org/10.1039/B314060C
Article type
Paper
Submitted
04 Nov 2003
Accepted
16 Feb 2004
First published
05 Mar 2004

Phys. Chem. Chem. Phys., 2004,6, 1482-1486

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Interactions between bovine serum albumin layers adsorbed on different substrates measured with an atomic force microscope

J. J. Valle-Delgado, J. A. Molina-Bolívar, F. Galisteo-González, M. J. Gálvez-Ruiz, A. Feiler and M. Rutland, Phys. Chem. Chem. Phys., 2004, 6, 1482 DOI: 10.1039/B314060C

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