Issue 11, 2005
From the journal:

Journal of Analytical Atomic Spectrometry

A continuous-flow dialysis system with inductively coupled plasma optical emission spectrometry for in vitro estimation of bioavailability†

Kunchit Judprasong,ab   Mathuros Ornthai,a   Atitaya Siripinyanonda  and  Juwadee Shiowatana*a  

* Corresponding authors

a Department of Chemistry, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok 10400, Thailand
E-mail: scysw@mahidol.ac.th
Fax: 66 2 3547151
Tel: 66 2 2015122

b On study leave from Institute of Nutrition, Mahidol University, Salaya, Putthamonthon, Nakorn Pathom 73170, Thailand
E-mail: nukjp@mahidol.ac.th
Fax: 66 2 4419344
Tel: 66 2 8002380

Abstract

A continuous-flow dialysis (CFD) method with on-line inductively coupled plasma optical emission spectrometric (ICP-OES) simultaneous multielement measurement for the study of in vitro mineral bioavailability was developed. The method was based on a simulated gastric digestion in a batch system followed by a continuous-flow intestinal digestion. The simulated intestinal digestion was performed in a dialysis bag placed inside a channel in a flowing stream of dialyzing solution (NaHCO3). The mineral concentrations in the dialysate were determined by ICP-OES using Y and Sc as internal standards. The pH of the dialysate was also monitored on-line to ensure pH changes similar to the situation in the gastrointestinal tract.The developed system was applied to determining the dialysability of five essential elements (Ca, Mg, P, Fe, Zn) for various kinds of foods, i.e., milk-based infant formula reference material (NIST SRM 1846), milk powder, kale, mungbean, chicken meat, jasmine rice, and Acacia pennata. The dialysis profiles of elements and pH change profiles can be useful in understanding the dialysis change and factors affecting dialysability. All studied elements were rapidly dialysed in the first 30 min of simulated intestinal digestion. It is expected that this system will be useful for estimation of dialysability and for studying the mutual effects of components in food.

Graphical abstract: A continuous-flow dialysis system with inductively coupled plasma optical emission spectrometry for in vitro estimation of bioavailability

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Article information

DOI
https://doi.org/10.1039/B508639F
Article type
Paper
Submitted
17 Jun 2005
Accepted
08 Sep 2005
First published
22 Sep 2005

J. Anal. At. Spectrom., 2005,20, 1191-1196

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A continuous-flow dialysis system with inductively coupled plasma optical emission spectrometry for in vitro estimation of bioavailability

K. Judprasong, M. Ornthai, A. Siripinyanond and J. Shiowatana, J. Anal. At. Spectrom., 2005, 20, 1191 DOI: 10.1039/B508639F

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