Issue 2, 2007

A linear dilution microfluidic device for cytotoxicity assays

Abstract

A two-layer polymer microfluidic device is presented which creates nine linear dilutions from two input fluid streams mixed in varying volumetric proportions. The linearity of the nine dilutions is conserved when the flow rate is held constant at 1.0 µl min−1 (R2 = 0.9995) and when it is varied from 0.5–16 µl min−1 (R2 = 0.9998). An analytical expression is presented for designing microfluidic devices with arbitrary numbers of linear dilutions. To demonstrate the efficacy of this device, primary human epidermal keratinocytes (HEK) were stained with nine dilutions of calcein, resulting in a linear spread of fluorescent intensities (R2 = 0.94). The operating principles of the device can be scaled up to incorporate any number of linear dilutions. This scalability, coupled with an intrinsic ability to create linear dilutions under a variety of operating conditions, makes the device applicable to high throughput screening applications such as combinatorial chemistry or cytotoxicity assays.

Graphical abstract: A linear dilution microfluidic device for cytotoxicity assays

Article information

Article type
Paper
Submitted
26 Jun 2006
Accepted
28 Sep 2006
First published
19 Oct 2006

Lab Chip, 2007,7, 226-232

A linear dilution microfluidic device for cytotoxicity assays

G. M. Walker, N. Monteiro-Riviere, J. Rouse and A. T. O'Neill, Lab Chip, 2007, 7, 226 DOI: 10.1039/B608990A

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