Issue 11, 2007

Self-assembled cellular microarrays patterned using DNA barcodes

Abstract

The successful integration of living cells into synthetic devices requires precise control over cell patterning. Here we describe a versatile platform that can accomplish this goal through DNA hybridization. Living cells functionalized with exogenous cell-surface DNA strands bind to cognate sequences of DNA printed on glass slides. Attachment via these “cell-adhesion barcodes” is rapid and specific, with close-packed arrays of cells forming within minutes. The biophysical properties of the system are characterized, and the technique is used to form complex cellular patterns with single-cell line widths and self-assembled cellular microarrays. Key advantages of DNA-directed cell binding include the ability to immobilize both adherent and non-adherent cells, to capture cells selectively from a mixed population, to tune the binding properties of the cells, and to reuse substrates prepared with widely available DNA printing technologies.

Graphical abstract: Self-assembled cellular microarrays patterned using DNA barcodes

Article information

Article type
Paper
Submitted
07 Jun 2007
Accepted
28 Aug 2007
First published
19 Sep 2007

Lab Chip, 2007,7, 1442-1448

Self-assembled cellular microarrays patterned using DNA barcodes

E. S. Douglas, R. A. Chandra, C. R. Bertozzi, R. A. Mathies and M. B. Francis, Lab Chip, 2007, 7, 1442 DOI: 10.1039/B708666K

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