Issue 3, 2009
From the journal:

Lab on a Chip

Absolute brightness of fluorescent microspheres

Isaac Finger,a   Scott Phillips,b   Elizabeth Mobley,a   Robert Tuckera  and  Henry Hess*a  

* Corresponding authors

a Department of Materials Science and Engineering, University of Florida, Gainesville, USA
E-mail: hhess@mse.ufl.edu
Fax: +1(352) 846-3355
Tel: +1(352) 846-3781

b ANDOR Technology PLC, South Windsor, CT, USA

Abstract

The absolute brightness of fluorescent particles, such as dye-containing nano- and microspheres or quantum dots, is a critical design parameter for many applications relying on fluorescence detection. The absolute brightness, defined as the ratio of radiant intensity of emission to illumination intensity of excitation, of nile-red fluorescent microspheres with a 1 micrometre diameter is measured to be 4.2 ± 1 × 10−16 m2/sr, and the implications for the design of kinesin motor protein-powered “smart dust” devices and the remote detection of fluorescence are discussed.

Graphical abstract: Absolute brightness of fluorescent microspheres

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Article information

DOI
https://doi.org/10.1039/B810219H
Article type
Technical Note
Submitted
17 Jun 2008
Accepted
02 Oct 2008
First published
10 Nov 2008

Lab Chip, 2009,9, 476-478

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Absolute brightness of fluorescent microspheres

I. Finger, S. Phillips, E. Mobley, R. Tucker and H. Hess, Lab Chip, 2009, 9, 476 DOI: 10.1039/B810219H

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