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Microfluidic & nanofluidic technologies for chemistry, physics, biology, and bioengineering
Paper
Lab Chip, 2009, 9, 3095 - 3099, DOI: 10.1039/b907309b
Detection of prostate specific antigen with nanomechanical resonators
Philip S. Waggoner, Madhukar Varshney and Harold G. Craighead
In this work, we use arrays of nanomechanical resonators to detect prostate specific antigen (PSA), a protein biomarker associated with prostate cancer. The surfaces of our very thin, trampoline-like devices are functionalised for immunospecific capture of PSA molecules, and the mass of bound material can be detected as a reduction in the resonant frequency. Fetal bovine serum was spiked with known concentrations of PSA, and in conjunction with a nanoparticle-based sandwich assay, concentrations as low as 50 fg mL-1, or 1.5 fM, could be detected from the realistic samples. The presence of non-specific proteins in the serum did not significantly affect the sensitivity of our assay.
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