Issue 9, 2007
From the journal:

Photochemical & Photobiological Sciences

Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers†

Sean Mathai,a   Damian K. Bird,a   Stan S. Stylli,b   Trevor A. Smith*a  and  Kenneth P. Ghigginoa  

* Corresponding authors

a School of Chemistry, The University of Melbourne, Victoria, Australia
E-mail: trevoras@unimelb.edu.au
Fax: +61-3-9347 5180
Tel: +61-3 8344 6272

b Department of Surgery, Royal Melbourne Hospital, Parkville, Australia

Abstract

Three porphyrin systems have been characterised for use in two-photon fluorescence imaging of biological samples. We have determined the two-photon absorption cross sections (σ2) of the di-cation, free-base and metallated forms of hematoporphyrin derivative (HpD), hematoporphyrin IX (Hp9) and a boronated protoporphyrin (BOPP) using the open-aperture Z-scan and the two-photon induced fluorescence (TPIF) techniques at an excitation wavelength of 800 nm. The insertion of either protons or a metal ion into the macrocycle is shown not to significantly influence the σ2 of the porphyrins. Two-photon time-resolved fluorescence images of C6 glioma cells transfected with a free-base form of the BOPP have been obtained as a function of the porphyrin concentration. These studies reveal a maximum useful porphyrin concentration for fluorescence imaging purposes of approximately 30 µg mL−1.

Graphical abstract: Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B705101H
Article type
Paper
Submitted
03 Apr 2007
Accepted
05 Jul 2007
First published
19 Jul 2007

Photochem. Photobiol. Sci., 2007,6, 1019-1026

Permissions

Request permissions

Two-photon absorption cross-sections and time-resolved fluorescence imaging using porphyrin photosensitisers

S. Mathai, D. K. Bird, S. S. Stylli, T. A. Smith and K. P. Ghiggino, Photochem. Photobiol. Sci., 2007, 6, 1019 DOI: 10.1039/B705101H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements