Issue 2, 2004
From the journal:

Analyst

Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry

Jorgelina C. A. Wuilloud,*a   Samuel R. Gratz,†a   Bryan M. Gamblea  and  Karen A. Wolnika  

* Corresponding authors

a US Food and Drug Administration, Forensic Chemistry Center, Cincinnati, Ohio 45237-3097, USA

Abstract

The purpose of the current study was to develop a LC-MSn method for the analysis of pyrrolizidine alkaloids (PAs) in comfrey. Published data presents an extensive list of PAs and their N-oxides present in comfrey. However, standards are not commercially available for any of the PAs typically present in comfrey. Those PAs that are not stereoisomers were readily resolved on a C18 column using a water–acetonitrile gradient as the mobile phase. The use of a selective technique, LC-MS/MS, allowed us to identify groups of PAs and their N-oxides, as well as identify the number of PAs present in each group, including those that were not completely resolved chromatographically.

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Article information

DOI
https://doi.org/10.1039/B311030C
Article type
Paper
Submitted
10 Sep 2003
Accepted
10 Dec 2003
First published
08 Jan 2004

Analyst, 2004,129, 150-156

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Simultaneous analysis of hepatotoxic pyrrolizidine alkaloids and N-oxides in comfrey root by LC-ion trap mass spectrometry

J. C. A. Wuilloud, S. R. Gratz, B. M. Gamble and K. A. Wolnik, Analyst, 2004, 129, 150 DOI: 10.1039/B311030C

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