Issue 9, 2004
From the journal:

Journal of Analytical Atomic Spectrometry

Determination of trace elements in serum samples by isotope dilution inductively coupled plasma mass spectrometry using on-line dialysis†

Maria Fernanda Giné,*a   Ana Cláudia S. Bellatoa  and  Amauri A. Menegáriob  

* Corresponding authors

a CENA-USP, Av. Centenário 303, C.P. 96, 13400-970 Piracicaba, SP, Brazil
E-mail: mfgine@cena.usp.br
Fax: 55 19 34294647
Tel: 55 19 34294636

b Centro de Estudos Ambientais, Universidade Estadual Paulista, Av. 24-A 1515, 13506-900 Rio Claro, SP, Brazil
E-mail: amenega@rc.unesp.br
Fax: 55 19 3534 0122
Tel: 55 19 3534 0122

Abstract

An on-line dialysis flow system coupled to inductively coupled plasma mass spectrometry to determine trace elements in serum samples by isotope dilution is presented. Isotope dilution was performed on samples incubated with enriched 65Cu, 66Zn, 77Se and 206Pb for 24 h at 36 °C prior to dialysis to quantified total element concentrations. The sample and acceptor solutions flowed through the dialysis unit with cellophane membrane placed in between the compartments. The serum sample (1 mL) was left to recycle in a closed path while the acceptor solution was continuously pumped along the dialyzer channel and through a cationic AG50W X-8 resin column. After 10 min, around 70% of Na, K and Cl migrate from the sample. Three replicate injections of 0.1 mL were performed for the clean sample after each separation step. The on-line coupling of the dialyzer to ICP-MS allowed isotope dilution for total element determination either in the cleaned sample or by eluting the cations retained in the resin to be carried out. Results demonstrated no matrix effects from alkaline elements or spectral interference from ArNa+ on 63Cu, ArCl+ on 77Se and 34SO2+ on 66Zn. The precision of isotope ratio measurements for Cu and Zn was around 1% and for Se and Pb was around 2.5%. The values found for the reference serum sample IMEP-17 were in good agreement with the certified values for Cu, Zn and Se.

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Article information

DOI
https://doi.org/10.1039/B403429P
Article type
Paper
Submitted
04 Mar 2004
Accepted
17 May 2004
First published
13 Aug 2004

J. Anal. At. Spectrom., 2004,19, 1252-1256

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Determination of trace elements in serum samples by isotope dilution inductively coupled plasma mass spectrometry using on-line dialysis

M. F. Giné, A. C. S. Bellato and A. A. Menegário, J. Anal. At. Spectrom., 2004, 19, 1252 DOI: 10.1039/B403429P

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