Issue 10, 2000
From the journal:

Analyst

Pulsed flow chemistry: a new approach to solution handling for flow analysis coupled with

Simon W. Lewis,*a   Paul S. Francis,a   Kieran F. Lim,a   Graeme E. Jenkinsb  and  Xue D. Wangc  

* Corresponding authors

a Centre for Chiral and Molecular Technologies, School of Biological and Chemical Sciences, Deakin University, Geelong, Victoria, Australia

b Precision Devices P/L, 44 Nelson Street, Shoreham, Victoria, Australia

c School of Chemical and Biomedical Sciences, Central Queensland University, Rockhampton, Queensland, Australia

Abstract

Novel instrumentation for flow analysis, coupled with chemiluminescence detection, and based on pulsed flow chemistry is described. The pulsed flow chemistry manifold allows for rapid and efficient mixing of chemiluminescent reagent with sample immediately in front of the detector, thus giving high precision (less than 1% RSD) and detectability, particularly for rapid, short-lived emissions. The instrument is also characterised by a compact size, high sampling frequency (120–300 samples per hour) and low reagent consumption (less than 500 μl per cycle). Other flow analysis modes (e.g., flow injection, continuous flow and stopped-flow) are available by simply altering software settings. The suitability of the system was shown by using chemiluminescence reactions of analytical significance, namely the oxidation of luminol with hexacyanoferrate(III) in the presence of 4-hydroxy-3-methoxybenzeneacetic acid (homovanillic acid), and the reduction of acidic potassium permanganate by morphine in the presence of polyphosphates. The latter reaction was used to determine morphine in aqueous process samples and the results obtained with the pulsed flow instrument displayed good agreement (mean difference of 1.8%) with those of a previously validated flow injection analysis method.

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B005118G
Article type
Paper
Submitted
26 Jun 2000
Accepted
10 Aug 2000
First published
18 Sep 2000

Analyst, 2000,125, 1869-1874

Permissions

Request permissions

Pulsed flow chemistry: a new approach to solution handling for flow analysis coupled with chemiluminescence detection

S. W. Lewis, P. S. Francis, K. F. Lim, G. E. Jenkins and X. D. Wang, Analyst, 2000, 125, 1869 DOI: 10.1039/B005118G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements