Volume 132, 2006
From the journal:

Faraday Discussions

A new approach for DNA detection by SERRS

Karen Faulds,*a   Ljiljana Fruk,a   David C. Robson,a   David G. Thompson,a   Alexis Enright,a   W. Ewen Smitha  and  Duncan Graham*a  

* Corresponding authors

a Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UK
E-mail: Duncan.graham@strath.ac.uk, Karen.faulds@strath.ac.uk
Fax: 0141 552 0876
Tel: 0141 548 2672

Abstract

A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering (SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.

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Article information

DOI
https://doi.org/10.1039/B506219E
Article type
Paper
Submitted
03 May 2005
Accepted
19 May 2005
First published
17 Oct 2005

Faraday Discuss., 2006,132, 261-268

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A new approach for DNA detection by SERRS

K. Faulds, L. Fruk, D. C. Robson, D. G. Thompson, A. Enright, W. Ewen Smith and D. Graham, Faraday Discuss., 2006, 132, 261 DOI: 10.1039/B506219E

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