Issue 15, 2007
From the journal:

Organic & Biomolecular Chemistry

Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids†

Hai Yu,a   Harshal A. Chokhawala,a   Ajit Varkib  and  Xi Chen*a  

* Corresponding authors

a Department of Chemistry, University of California-Davis, One Shields Avenue, Davis, CA, USA
E-mail: chen@chem.ucdavis.edu
Fax: +1 (1)530 752 8995
Tel: +1 (0)530 754 6037

b Departments of Medicine and Cellular & Molecular Medicine, University of California, San Diego, La Jolla, CA, USA

Abstract

Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate–protein conjugates containing base-sensitive groups.

Graphical abstract: Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids

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Article information

DOI
https://doi.org/10.1039/B706507H
Article type
Paper
Submitted
30 Apr 2007
Accepted
11 Jun 2007
First published
27 Jun 2007

Org. Biomol. Chem., 2007,5, 2458-2463

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Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids

H. Yu, H. A. Chokhawala, A. Varki and X. Chen, Org. Biomol. Chem., 2007, 5, 2458 DOI: 10.1039/B706507H

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