Issue 11, 2008
From the journal:

Organic & Biomolecular Chemistry

Dendronised block copolymers as potential vectors for gene transfection†

Tony J. Wigglesworth,a   Francisco Teixeira,  Jr.,b   Fabian Axthelm,b   Sara Eisler,a   Noemi S. Csaba,c   Hans P. Merkle,c   Wolfgang Meierb  and  François Diederich*a  

* Corresponding authors

a Laboratorium für Organische Chemie, ETH-Zürich, Zürich, Switzerland
E-mail: diederich@org.chem.ethz.ch
Fax: +41 44 632 1109
Tel: +41 44 632 2992

b Department of Chemistry, University of Basel, Basel, Switzerland
E-mail: wolfgang.meier@unibas.ch

c Institut für Pharmazeutische Wissenschaften, ETH-Zürich, Zürich, Switzerland
E-mail: hmerkle@pharma.ethz.ch

Abstract

A series of block copolymers containing a dendronised cationic block for efficient DNA binding and a poly(ethylene glycol) block for encapsulation of the complex were synthesised in a modular fashion using a combination of click chemistry and ring-opening metathesis polymerisation. DNA binding experiments, investigated using gel electrophoresis, dynamic light scattering and transmission electron microscopy, showed that all polymers prepared in this study strongly complex DNA and self-assemble into polyion complex micelles with apparent hydrodynamic radii ranging from 20–120 nm at physiological pH (7.4). The in vitro transfection efficiency and toxicity of these potential non-viral vectors were also evaluated in HeLa‡ cells using plasmid DNA encoding for green fluorescent protein as the reporter gene.

Graphical abstract: Dendronised block copolymers as potential vectors for gene transfection

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Article information

DOI
https://doi.org/10.1039/B802808G
Article type
Paper
Submitted
19 Feb 2008
Accepted
06 Mar 2008
First published
07 Apr 2008

Org. Biomol. Chem., 2008,6, 1905-1911

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Dendronised block copolymers as potential vectors for gene transfection

T. J. Wigglesworth, F. Teixeira, F. Axthelm, S. Eisler, N. S. Csaba, H. P. Merkle, W. Meier and F. Diederich, Org. Biomol. Chem., 2008, 6, 1905 DOI: 10.1039/B802808G

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