Issue 8, 2007
From the journal:

Analyst

High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis†

G. Seguí-Lines,a   J. M. A. Gavina,a   J. C. D'Amarala  and  P. Britz-McKibbin*a  

* Corresponding authors

a Department of Chemistry, McMaster University, 1280 Main Street West, Hamilton, ON, Canada
E-mail: britz@mcmaster.ca
Fax: +1-905-522-2509

Abstract

Dynamic ligand exchange-affinity capillary electrophoresis (DLE-ACE) is introduced as a convenient platform for assessing the conformational stability and relative affinity of a holoprotein to different ligands without off-line sample pretreatment, since ligand exchange and protein unfolding processes are integrated in-capillary during electromigration.

Graphical abstract: High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis

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Article information

DOI
https://doi.org/10.1039/B705469F
Article type
Communication
Submitted
11 Apr 2007
Accepted
04 Jun 2007
First published
12 Jun 2007

Analyst, 2007,132, 741-744

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High-throughput screening of holoprotein conformational stability by dynamic ligand exchange-affinity capillary electrophoresis

G. Seguí-Lines, J. M. A. Gavina, J. C. D'Amaral and P. Britz-McKibbin, Analyst, 2007, 132, 741 DOI: 10.1039/B705469F

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