Issue 9, 2008
From the journal:

Lab on a Chip

Cell characterization using a protein-functionalized pore†

Andrea Carbonaro,a   Swomitra K. Mohanty,a   Haiyan Huang,b   Lucy A. Godleyc  and  Lydia L. Sohn*a  

* Corresponding authors

a Dept. of Mechanical Engineering, University of California at Berkeley, 5118 Etcheverry Hall, Mail Stop 1740, Berkeley, CA, USA
E-mail: sohn@me.berkeley.edu
Fax: 510-643-5599
Tel: 510-642-5434

b Dept. of Statistics, University of California, Berkeley, CA, USA

c Section of Hematology/Oncology, Dept. of Medicine, The University of Chicago, IL, USA

Abstract

We demonstrate a highly-sensitive and label-free method for characterizing cells based on cell-surface receptors. The method involves measuring a current pulse generated when an individual cell passes through an artificial pore. When the pore is functionalized with proteins, specific interactions between a cell-surface marker and the functionalized proteins retard the cell, thus leading to an increased pulse duration that indicates the presence of that specific biomarker. For proof-of-principle, we successfully screened murine erythroleukemia cells based on their CD34 surface marker in both a single and mixed population of cells. Further, we developed a unified constrained statistical model for estimating the ratios of cells in a mixed population. Finally, we demonstrated our ability to screen a small number of cells (hundreds or less) with high accuracy and sensitivity. Overall, our pore-based method is broadly applicable and, in the future, could provide a full range of in vitrocell-based assays.

Graphical abstract: Cell characterization using a protein-functionalized pore

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Article information

DOI
https://doi.org/10.1039/B801929K
Article type
Paper
Submitted
04 Feb 2008
Accepted
26 Jun 2008
First published
25 Jul 2008

Lab Chip, 2008,8, 1478-1485

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Cell characterization using a protein-functionalized pore

A. Carbonaro, S. K. Mohanty, H. Huang, L. A. Godley and L. L. Sohn, Lab Chip, 2008, 8, 1478 DOI: 10.1039/B801929K

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