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Microfluidic & nanofluidic technologies for chemistry, physics, biology, and bioengineering



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Paper

Lab Chip, 2009, 9, 2010 - 2015, DOI: 10.1039/b821690h

DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array

Erik S. Douglas, Sonny C. Hsiao, Hiroaki Onoe, Carolyn R. Bertozzi, Matthew B. Francis and Richard A. Mathies

A microdevice is developed for DNA-barcode directed capture of single cells on an array of pH-sensitive microelectrodes for metabolic analysis. Cells are modified with membrane-bound single-stranded DNA, and specific single-cell capture is directed by the complementary strand bound in the sensor area of the iridium oxide pH microelectrodes within a microfluidic channel. This bifunctional microelectrode array is demonstrated for the pH monitoring and differentiation of primary T cells and Jurkat T lymphoma cells. Single Jurkat cells exhibited an extracellular acidification rate of 11 milli-pH min-1, while primary T cells exhibited only 2 milli-pH min-1. This system can be used to capture non-adherent cells specifically and to discriminate between visually similar healthy and cancerous cells in a heterogeneous ensemble based on their altered metabolic properties.

Graphical abstract image for this article (ID: b821690h)