RSC Publishing


Publishing

 

Cover image for Lab on a Chip, select for current issue

Lab on a Chip

Microfluidic & nanofluidic technologies for chemistry, physics, biology, and bioengineering



Subscribers

Non-subscribers

Free access



Communication

Lab Chip, 2009, 9, 2281 - 2285, DOI: 10.1039/b907430a

An integrated microfluidic device for large-scale in situ click chemistry screening

Yanju Wang, Wei-Yu Lin, Kan Liu, Rachel J. Lin, Matthias Selke, Hartmuth C. Kolb, Nangang Zhang, Xing-Zhong Zhao, Michael E. Phelps, Clifton K. F. Shen, Kym F. Faull and Hsian-Rong Tseng

An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

Graphical abstract image for this article (ID: b907430a)