Issue 24, 2009
From the journal:

Soft Matter

Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells†

Sally Hopkins,ab   Steven R. Carter,a   John W. Haycock,b   Nigel J. Fullwood,c   Sheila MacNeilb  and  Stephen Rimmer*a  

* Corresponding authors

a Polymer and Biomaterials Chemistry Laboratories, Department of Chemistry, University of Sheffield, Sheffield, South Yorkshire, UK
E-mail: s.rimmer@sheffield.ac.uk
Fax: +44 (0)1977 618498
Tel: +44 (0)114 222 0000

b Department of Engineering Materials, The Kroto Research Institute, University of Sheffield, Sheffield, UK

c Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK

Abstract

We describe the first example of particulate materials that can detach cultured cells and then release them intact in a temperature controlled manner. Topologically open microgels composed of water swollen highly branched polymers prepared from poly(N-isopropylacrylamide) (PNIPAM) were modified with a cell-adhesive peptide (GRGDS) to produce particles for gently detaching and then transferring cultured cells to new substrates. The particles bind to cell surface integrins on both dermal fibroblasts and endothelial cells and at temperatures above the lower critical solution temperature (34 °C) remove cells from their normal culture substrates. Brief (45 min) cooling of the resultant particle–cell dispersion to beneath 34 °C releases the cells to grow on new substrates. This avoids the need for trypsinisation to detach cells or centrifugation to collect cells post-detachment and offers a flexible approach to cell detachment and transport which is compatible with normal cell culture methodologies.

Graphical abstract: Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells

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Article information

DOI
https://doi.org/10.1039/B909656F
Article type
Paper
Submitted
15 May 2009
Accepted
21 Aug 2009
First published
19 Oct 2009

Soft Matter, 2009,5, 4928-4937

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Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells

S. Hopkins, S. R. Carter, J. W. Haycock, N. J. Fullwood, S. MacNeil and S. Rimmer, Soft Matter, 2009, 5, 4928 DOI: 10.1039/B909656F

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