Issue 15, 2010
From the journal:

Soft Matter

Image correlation spectroscopy as a tool for microrheology of soft materials†

Nicholas Agung Kurniawan,a   Chwee Teck Limabc  and  Raj Rajagopalan*ade  

* Corresponding authors

a NUS Graduate School for Integrative Sciences and Engineering, Singapore
E-mail: raj@nus.edu.sg

b Department of Mechanical Engineering, National University of Singapore, Singapore

c Division of Bioengineering, National University of Singapore, Singapore

d Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore

e Chemical and Pharmaceutical Engineering Program, Singapore-MIT Alliance, National University of Singapore, Singapore

Abstract

Image correlation spectroscopy (ICS) has been mostly used to quantify spatiotemporal events in biological experiments. Here, we develop and demonstrate the use of ICS for microrheology (μR) of complex, viscoelastic materials. A special technique for extracting mean-squared displacements (MSD) of probe particles in the samples from image correlation data is developed. The method (ICS-μR) is tested on Newtonian (glycerol solutions in water) and complex fluids (poly(ethylene oxide) (PEO) solutions). Accurate measurements of the viscosities of the Newtonian fluids (equivalently, the diffusion coefficients of the probe particles) as well as the storage and loss moduli of the PEO solutions could be obtained. The possibility to combine spatiotemporal assessment and time- and length-scale dependent microrheological measurements from images of fluorescent molecules makes ICS-μR a prospective tool in many biophysical applications. Further, the method developed for extracting MSD from image (or intensity) correlation measurements can be applied to other microrheological techniques to provide improved accuracy of rheological measurements.

Graphical abstract: Image correlation spectroscopy as a tool for microrheology of soft materials

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Article information

DOI
https://doi.org/10.1039/C002265A
Article type
Paper
Submitted
02 Feb 2010
Accepted
19 Apr 2010
First published
10 Jun 2010

Soft Matter, 2010,6, 3499-3505

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Image correlation spectroscopy as a tool for microrheology of soft materials

N. A. Kurniawan, C. T. Lim and R. Rajagopalan, Soft Matter, 2010, 6, 3499 DOI: 10.1039/C002265A

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